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A Comprehensive Method of Identifying Heat Shock Proteins (HSPs)

Pielesz A, Machnicka A, Binias D and Sarna E

Because no model will ever completely replicate clinical human wound healing, it is essential that the model utilized be selected with care. Anatomically and physiologically, poultry skin is similar to human skin in many respects. Therefore, organic chicken skin (an ex-vivo burninjured skin model) was analysed in this study. Acetate electrophoresis (CAE), microbiological procedure, Fourier-transform infrared spectrometry (FTIR) and scanning electron microscopy analysis (SEM) were all carried out after heating samples of model chicken skin to a temperature simulating a burn incident and stimulating the release of Heat Shock Proteins (HSPs). Aggregates of smaller molecular weight, HSP37 proteins, were isolated by cellulose acetate electrophoresis. FTIR tests revealed that heating a dry organic chicken skin to boiling point leads to the production of β-sheet aggregates, which are the response of protein to thermal shock. Aggregates of HSP37 are produced in thermal injury and not all the antimicrobial activity of the skin is lost in this model. So, antimicrobial peptides found in the burnt skin, HSP proteins were confirmed by microscopic, microbiological, electrophoretic and spectroscopic examination.