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Evaluation of Antimicrobial Effects of Three Medicinal Plants in South of Iran against the Staphylococcus Aureus, Pseudomonas Aeruginosa and Escherichia Coli.

Mustafa Baloch and Reza Ranjbar

Nowadays, natural treatment of bacterial diseases using substances which are herbal is of utmost importance. In the present study effects of three antibacterial medicinal plants in south of Iran against the separated Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli from Clinical samples and standard strains was investigated. Hydro-alcoholic extracts of Ziziphora tenuior, Origanum Vulgare and Mentha longifolia were prepared after being dried at shade. In the following the antibacterial activity of the extracts against separated Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli from clinical samples of the Shiraz hospitals and standard samples using disk agar diffusion method was investigated and the drug resistance in the studied samples was conducted using the standard Kirby-Bauer method. The growth of the all studied Staphylococcus aureus and Escherichia coli was stopped by hydro-alcoholic extracts of Mentha longifolia, Ziziphora tenuior and Origanum Vulgare but Pseudomonas aeruginosa showed growth zone of inhibition only against Origanum Vulgare. From statistical viewpoint Origanum Vulgare had also the most antimicrobial effect against Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli in the all studied samples (P<0.05). Extracts of Mentha longifolia, Origanum Vulgare and Ziziphora tenuior have a potent antibacterial effect against the gram-positive and gram-negative. But on the nosocomial pathogen, Pseudomonas aeruginosa, only Origanum Vulgare has growth inhibitory effect.