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A Rapid Sensitive Detection Method by Plate Assay for Catalase Activity from Bacterium Acinetobacter calcoaceticus AV6

Vijayakumar Arul Doss, Murugesan Subban, Jayapal Jmbulingam, Panneerselvam Annamalai and Kalaichelvan PT

Bacterium exhibiting extra-cellular catalase activity was isolated from lime stone soil. Catalase is an essential component of the cell to cope up with oxidative stress. This study deals with simple detection method by plate assay for catalase activity from bacterium Acinetobacter calcoaceticus AV-6. Rapid assay method involves plating the supernatant on the substrate bound agar with the dye. This assay gave result within 60 s, producing a zone around the well. This enzyme was extracted from stationary phase at 36 h. The molecular weight of the protein was determined to be approximately 60 kDa. Sequencing studies were carried out and the strain Acinetobacter calcoaceticus AV6 was partially sequenced. The results imply the significance of this detection method in industries.