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Taxonomic Identification and Phylogenetic analysis of Nematode Parasites Using 18S rDNA and 28S rDNA

Sangeeta Pal and Hirdaya Shanker Singh

Molecular markers have often been used for taxonomic identification and phylogenetic analyses in different species groups. Evolution of rDNA is relatively independent of changes in morphology, and analyses of these genetic data have been shown to provide good phylogenetic resolution. So, it was decided to perform a phylogenetic analysis on species of the genus Leidynema appendiculata, Hammerschmidtiella indicus and Schwenkiella icemi based on ribosomal DNA (rDNA) sequences. During the present study, a partial fragment of the small subunit ribosomal RNA gene (SSU rRNA) and large subunit ribosomal RNA gene (LSU rRNA) was sequenced for purposes of molecular identification. In the present study, phylogenetic relationships of species of the genus were investigated using nucleotide sequences of the region of 28S rDNA and 18S rDNA. Phylogenetic analyses were performed for primary sequence data as well as using neighbour-joining and maximumparsimony approaches.