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Protein Expression of Flavonol Synthase from Fragrant Wild Cyclamen (Cyclamen Purpurascens)

Tomomi Iwasaki, Riho Mikami and Yusuke Akita

To aid the development of DNA markers and new flower-color mutants, we previously isolated and analyzed the flavonoid biosynthesis-related genes involved in flower coloration in fragrant wild cyclamen (Cyclamen purpurascens). Two flavonol synthase genes (CpurFLS1 and CpurFLS2) were subsequently isolated and CpurFLS2 found to be related to flower coloration. As a next step, in vitro observations of enzymatic activity are necessary; therefore, expression analysis of CpurFLS2 protein was conducted. In this study, we provide the optimal conditions for recombinant soluble CpurFLS2 protein production in Escherichia coli.