Sumalatha Avvaru
Tissue culture is a technique for immunization and separation of tissues in manufactured medium under in vitro condition. It is a gathering of test strategies by utilizing organs, tissues and cell in a simulated medium under in vitro aseptic environment. Banana is real natural product crop in India as Maharashtra stands first in banana creation and efficiency in India. By utilizing miniaturized scale spread system these plants are refined in labs and this technique is a vegetative proliferation. Ordinarily banana is developed by utilizing suckers. In agriculturist's thought, tissue refined plants are getting to be mainstream. This strategy produces infection free plant stocks and is spread by meristem tip culture. In meristem tip culture there is a partition of undifferentiated plant cells which are not valuable cells from shoot tips. Steps required in meristem tip society are: selection of explants, readiness and disinfection of explant, preparation and cleansing of society media, inoculation of explant, hatching of explant in development rooms with looked after temperatures, sub refined of explants, hardening and so on. Banana is imperative organic product crop with 97.5 million tons of generation everywhere throughout the world. In India as it backings work of a great many individuals. Banana development possesses around 20% region among the aggregate region under yield in India. The vast majority of banana yields are developed by planting suckers.