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New Insights on Cistus salviifolius L. Micropropagation

Rogerio Louro, Augusto Peixe and Celeste Santos-Silva

One of the major concerns in the establishment of any mycorrhization program is ensuring the mass production of sterile, consistent and standardized plant material. In the present study, a successful protocol for micropropagation of Cistus salviifolius L. was developed. The process was initiated from nodal segments excised from mature C. salviifolius plant selected due to its mycorrhizal capacities. Murashige and Skoog basal medium supplement with gibberellic acid (0.5 mg/L) and of 6-Benzylaminopurine (0.5 mg/L) was the best medium for proliferation purposes and successful rooting was achieved with the same basal medium supplemented with Indole-3-butyric acid (0.5 mg/L). The proposed methodology represent a novelty because it allowed the rapid multiplication of C. salviifolius starting from mature explants, here reported for the first time, using lower plant growth regulators concentrations than the previously reported for this particular Cistus species