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Micro-Tuberization of Four Potato (Solanum tuberosum L.) Cultivars Through Tissue Culture

Sayed Husain, Syed Abrar Hussain Shah, Syed Asghar, Nazim Hussain, Naushad Ali, Imtiaz Hussain, Saima Rafiq, Sadiq Shah, Muhammad Imtiaz, Muhammad Ali, Fazal Jala, Zia-ullah and Muhammad Rashid

An experiment for the micro-tuberization of four potato cultivars through tissue culture on different sucrose concentration in MS media was conducted at tissue culture lab Abbottabad, Pakistan. Four cultivars (Desiree, Cardinal, Ultimus and Astrix) and MS media supplemented with four levels of sucrose (20 gl-1, 30 gl-1, 40 gl-1 and 50 gl-1) were used in the experiment. All cultivars showed best performance in MS media supplemented with 40 gl-1 of sucrose in respect of all parameters such as micro-tubers formation, days to micro-tubers harvest, number of micro- tubers plantlet-1, micro-tuber diameter (cm), micro-tuber weight (g) and number of nodes in micro-tuber. MS media containing 50 gl-1 sucrose showed less effect in all the underline parameters under in vitro condition. Among the four different potato cultivars, astrix performed better followed by desiree in all under study parameters under in vitro condition.