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Isolation of Quercetin and Kaempferol From Azadirachta Indica Leaves and Its Synergistic Action Against Clinically Isolated Multi Drug Resistant Strains MRSA

Shafqat Ullah, Rehman Ullah Khan1, Shamsur Rehman Afridi1, Asad Ullah2, Arshad Hussain2 and Khaliq-ur-Rehman

The aim of this study was to isolate the two major flavonoids Quercetin and kaempferol by P-HPLC from Azadirachta Indica leaves and its activity against clinical isolated MRSA strains. The flavonoids were separated and identified by analytical column, while isolated by preparative column Intersil ODS-3 C-18 and its composition as in analytical procedure. Antibiotics (Cefixime, Ceftriaxone, Erythromycin, Ciprofloxacin, Cephradine, Methicillin, Ampicillin, Levoflaxacin and Amoxicillin) and flavonoids (quercetin, kaempferol and combination of quercetin + kaempferol) were evaluated against clinically isolated MRSA. The results showed that the Cefixime, Ceftriaxone, Ciprofloxacin, Cephradine, Methicillin, Ampicillin and Amoxicillin were found inactive against clinical isolated MRSA when tested alone, but when combine with quercetin, kaempferol and Q+K showed valuable anti-MRSA activity, while tested flavonoids showed were found to be no impact on Erythromycin and causing reduction in Levoflaxacin and Ciprofloxacint.