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Development of Transgenic Tomato Expressing Antimicrobial Peptide Gene through Agrobacterium-Mediated Transformation

Meenakshi S, Sukhada Mohandas, Riaz Mahmood

Agrobacterium-mediated transformation was developed for tomato plant (cv. Arka vikas) using Antimicrobial peptide gene under the control of CaMV 35S promoter in pCAMBIA 2301 vector. Protocol was optimised by various parameters influencing transformation. Hypocotyledonary explants where treated with Agrobacterium 0.4 O.D 600 for 2 min and selected on kanamycin 100 mg l-1. 59 shoots regenerated initially on MS media containing BAP – 2.0 mg l-1 and TDZ – 1.0 mg l-1. 13 plants were finally survived on hardening and screened for the presence of gene by PCR. 360 bp of gene amplification confirmed the presence of gene in 8 plants. Transformation frequency of 11 % was observed.