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Development and Validation of Spectroscopic Method for Simultaneous Estimation of Allopurinol and �?±-Lipoic Acid in Combination Tablet.

Darshan J Patel, Vineet C Jain, and Hasumati A Raj

A simple, accurate and precise spectroscopic method was developed for simultaneous estimation of Allopurinol and α-Lipoic Acid in combination tablet dosage form using AUC & Absorbance correction method. In Area Under Curve and Absorption correction method,α-Lipoic Acid has less Absorbance compare to Allopurinol for 10 to 50 ppm so in this method we consider area under curve at 310 nm to 390 nm in case of α-Lipoic Acid which found linear in given range of concentration and there is no interference of Allopurinol in this region because it has no absorbance in this region. Allopurinol is estimated by absorption correction method at 250 nm. Methanol used as solvent. Linearity was observed in the concentration range of 10-50 μg/ml for both Allopurinol and α-Lipoic Acid with correlation coefficient 0.9998 and 0.9999 for Allopurinol and α-Lipoic Acid respectively. Mean recovery were found to be 101.35% and 101.41% for Allopurinol and α-Lipoic Acid, respectively. The proposed method was successfully applied for the simultaneous estimation of both drugs in combination tablet.