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Critical Factor That Governs a Successful TLC/ HPTLC Analysis of Herbal Medicinal Products (HMPS).

Rahul Kasar, Ashish Gogia, Kartik Shah, Vetriselvan and Chinmay Anand

Chromatography is one of the fast emerging tools by which the quality control and fingerprint of herbs can be maintained. Using this technique, the identification of various chemical markers of the herbal drugs can be easily done and it also helps to identify the same herbs in combination. Application of TLC/HPTLC methodology in testing of phytoconstituents from individual herbal drugs and fingerprint characteristic of the herbal plants are reviewed in this paper. Popularity of TLC/HPTLC analytical method for analysis of herbal drugs due to economic, rapid, simultaneously screening of large number of herbal samples and less time consuming methods. The different mobile phase, spraying reagent, property of herbal drugs and its phytoconstituents, TLC/HPTLC plates, trouble shooting of HPTLC, different developing solvents and chromatograms are pointed out in this paper.