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Application of Chloramine-T, Methylene Blue in the Assay of Mesna in Tablets, Injections and Wastewater Samples

Nief Rahman Ahmed, Lujein Mohammad Kasim and Muna Sobhi Abdullah

A new, accurate, sensitive and simple indirect spectrophotometric method for the estimation of sodium 2-mercaptoethanesulfonate (mesna) has been developed. This procedure base on the drug oxidation with excess Chloramine-T in acidic solution, followed by the reaction of the excess oxidants with methylene blue, and read the absorbance at 664 nm .The absorbance results increased with increasing concentration of mesna .The calibration curve was linear at the range 0.1-2.0 ppm. Apparent molar absorbance, Sandell’s sensitivity values were 7.832×10⁴ Lmol^-1cm^-1. And 47 ngcm^-2, respectively. The (RSD) of this method was less than 1.8 and average recovery (accuracy) is 100 ± 0.95. The method applied successfully for estimation of mesna in waste water sample and in (tablets and injections pharmaceutical formulations).