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Antioxidant activities of the unripen and ripen Citrus aurantifolia of Assam

Sony Kumari, Neelanjana Sarmah, A.K. Handique

The in-vitro antioxidant activities of juices of both unripen and ripen Citrus aurantifolia (Assamese: kaji nemu) was determined. Juices were extracted from fresh ripen and unripen fruits by mechanical squeezing and were used for the present study. The antioxidant activity of juice was evaluated by using the free radical scavenging activity of 1, 1-diphenyl-2-picrylhydrazyl radical (DPPH), total antioxidant capacity (TAC) by the phosphomolybdenum method. pH and total acidity was also determined. Unripen juices showed more antioxidant scavenging activity as compared to ripen juices. The lower antioxidant activity of ripe fruit juices could be due to the possible reduction in the ascorbic acid and total phenolic content during ripening. The total antioxidant capacity in terms of ascorbic acid was also determined. Comparison was done for both types of juices. Our study reveals that juices of unripen and ripen Citrus aurantifolia possess strong antioxidant activity to provide protection against free radicals induced damage to biomolecules.