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Analytical Method Development and Validation of Azelnipidine and Telmisartan by RP HPLC Method

Vangallu Spandana*, Siddartha

A simple, Accurate, precise method was developed for the simultaneous estimation of the Azelnipidine and Telmisartan in tablet dosage form. Chromatogram was run through standard symmetry C18 (4.6 x 150 mm, 5 mm). Mobile phase containing Buffer 0.01 N KH₂PO: Acetonitrile taken in the ratio 45:55% v/v was pumped through column at a flow rate of 1 ml/min. Optimized wavelength selected was 290 nm. Retention time of Azelnipidine and Telmisartan were found to be 2.131 min and 2.593 min. %RSD of the Azelnipidine and Telmisartan were and found to be 0.8 and 0.6 respectively. %Recovery was obtained as 99.59% and 99.94% for Azelnipidine and Telmisartan respectively. LOD, LOQ values obtained from regression equations of Azelnipidine and Telmisartan were 0.86, 2.60 and 0.09, 0.29 respectively. Regression equation of Azelnidipine is y=12533 x +10387 and y=9061 x +183.8 of Telmisartan. Retention times were decreased and that run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.