Joseph Ahaneku*
A multitude of PCR based diagnostic techniques are available in modern plant pathology. A researcher should have a concept of the target sequences that could be used in a DNA assay to detect and identify a plant pathogen. As a result, the data generated by phytopathogen genome sequencing must be accessible. Because the primers ensure the specificity of PCR, they must be chosen and designed correctly for any PCR analysis to be successful. The selection of primers is the initial step in PCR diagnosis.
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