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A Short Note on Paper Chromatography and Its Types

Zheng Chang

Two-dimensional chromatography is a type of paper chromatography that includes utilizing two solvents and rotating the paper 90 degrees in between. This is useful for distinguishing complex combinations of comparable polarity chemicals, such as amino acids. There are three parts to the setup. Due to capillary action, the mobile phase is a solution that travels up the stationary phase. The mobile phase is usually a mixture of non-polar organic solvents, while the stationary phase is water, which is a polar inorganic solvent. The stationary phase, water, is supported here by paper