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A Review on High Performance Liquid Chromatography

Spoorthy N

Several liquid chromatographic methods are developed for a systematic analysis of various degradation products. High-performance liquid chromatography is the most widely used analytical separation technique because of its reproducibility sensitivity and suitability for separating nonvolatile species which makes the method ideal for accurate quantitative determinations. A reversed-phase HPLC method with UV detection is developed for simultaneous separation and quantitation of organic acids and neutral degradation products present in the corn stover hydrolysate. On the other hand inorganic ions and some organic anions which are present in the water extractive from corn stover are separated and quantitated by a developed ion chromatographic method with conductivity detection. Sugars and alditols are also determined using high-performance anion chromatography with pulsed amperometric detection